MAPK3 Antibody

• Catalog Number: orb1253726
• Category: Antibodies
• Description: MAPK3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: p44/42 MAP Kinase (Phospho-Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (F-L-T (p) -E-Y) derived from Human p44/42 MAP Kinase.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42, 44 kDa
• Target: MAPK3
• UniProt ID: P27361
• NCBI: NP_001035145.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KD
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA for the indicated times, using p44/42 MAP Kinase (Phospho-Thr202).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Thr202). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear staining using p44/42 MAP Kinase (Phospho-Thr202).