MAP2K1 Antibody

• Catalog Number: orb1239447
• Category: Antibodies
• Description: MAP2K1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: MEK1/2 antibody was raised against a 15 amino acid peptide near the center of human MEK1.The immunogen is located within amino acids 240 - 290 of MEK1/2.
• Concentration: 1 mg/mL
• Dilution range: MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 43 kDa Observed: 44 kDa
• Target: MAP2K1
• UniProt ID: Q0VD16
• NCBI: NP_002746
• Storage: MEK1/2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: MEK1/2 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Mitogen-activated protein kinase kinase 1, MAP2K1,
• Note: For research use only
• Application notes: MEK1/2 antibody can be used for detection of MEK1/2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MEK1/2 in human brain tissue lysate with MEK1/2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 5 µg/mL.

Immunofluorescence of MEK1/2 in mouse brain tissue with MEK1/2 antibody at 20 µg/mL.