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Catalog Number | orb764230 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to MEK-1/2 (phospho-Ser218/222) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser217. AA range:189-238 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 48 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-MAP2K1 antibody, anti-MEK1 antibody, anti-PRK Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, MEK-1/2 (phospho Ser218/222) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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