Lck antibody

• Catalog Number: orb43898
• Category: Antibodies
• Description: Mouse Monoclonal to Lck.
• Clonality: Monoclonal
• Clone Number: LCK-01
• Tested applications: FC, ICC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: LCK
• Entrez: 3932
• UniProt ID: P06239
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Lck antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck; Lane 2: HEK-293T cell line transfected with Lck; Lane 3: HEK-293T cell line (non-transfected).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LCK (LCK-01) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 positive LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LCK (LCK-01) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LCK (LCK-01) purified antibody (concentration in sample 9 µg/ml, GAM APC).