LC3B Antibody

• Catalog Number: orb1294361
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LC3B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Dilution range: Western Blot 1:1000 – 1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:200
• Conjugation: Unconjugated
• Target: LC3B
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

LC3B antibody at 1/1000 dilution. Lysates/proteins at 50 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LC3B with orb1294361 at 1/200.

Immunohistochemical analysis of paraffin embedded Human colorental and liver cancer tissue labeling LC3B with orb1294361 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Kidney cancer tissue labeling LC3B with orb1294361 at 1/100.

LC3B antibody at 1/1000 dilution. Lysates/proteins at 50 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4°C.

All lanes: Anti-LC3B antibody at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294361 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294361 anti-LC3B antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human testis and breast cancer tissue labeling LC3B with orb1294361 at 1/100.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294361 anti-LC3B antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.