LC3 antibody

• Catalog Number: orb97657
• Category: Antibodies
• Description: Mouse monoclonal antibody to LC3
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 166AT1234
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1 k
• Dilution range: WB: 1:1000, IF/ICC: 1:200, IHC-P: 1:50-100
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Target: This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
• UniProt ID: Q9H492, Q9GZQ8
• NCBI: NP_115903.1, NP_852610.1
• Alternative names: anti ATG8F antibody, anti Autophagy-related protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Graham, Christopher D. et al. Tamoxifen Induces Cytotoxic Autophagy in Glioblastoma J Neuropathol Exp Neurol, 75, 946-954 (2016)

PubMed: 27516117

Western blot analysis of Hela cell lysatesusing MAP1LC3A antibody (primary antibody dilution at: 1:1000)

Immunofluorescense analysis of U251 cells using MAP1LC3A antibody (dilution of primary antibody at 1:25)

Immunofluorescense analysis of U251 cells using MAP1LC3A antibody (antibody dilution at 1:25)

Immunofluorescense analysis of U251 cells using MAP1LC3A antibody (primary antibody dilution at: 1:200)

Immunohistochemical staining of paraffin embedded human muscle tissue using MAP1LC3A antibody (primary antibody dilution at: 1:50-100)