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Catalog Number | orb1267693 |
---|---|
Category | Antibodies |
Description | KLF9 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Predicted Reactivity | Mouse, Porcine |
Reactivity | Human, Rat |
Isotype | Rabbit Ig |
Immunogen | This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 27 kDa |
Target | KLF9 |
UniProt ID | Q13886 |
NCBI | Q13886 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Krueppel-like factor 9, Basic transcription elemen Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in WiDr cell line lysates (35 ug/lane).
Fluorescent confocal image of U251 cell stained with KLF9 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF9 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.
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