IRF3 Antibody

• Catalog Number: orb1824663
• Category: Antibodies
• Description: Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-Alpha and beta) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-Alpha and interferon-beta genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3gammap48 and IFN consensus sequence-binding protein (ICSBP).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-IRF3-4D7
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length human IRF3 protein was used as the immunogen for the Interferon regulatory factor 3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Interferon regulatory factor 3 antibody is available for research use only.
• UniProt ID: Q14653
• Storage: Aliquot the Interferon regulatory factor 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human HeLa cells with Interferon regulatory factor 3 antibody (clone PCRP-IRF3-4D7) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

IHC staining of FFPE human tumor of unknown origin with Interferon regulatory factor 3 antibody (clone PCRP-IRF3-4D7). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Interferon regulatory factor 3 antibody (clone PCRP-IRF3-4D7) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using Interferon regulatory factor 3 antibody (clone PCRP-IRF3-4D7). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.