IRF3 Antibody

• Catalog Number: orb2636911
• Category: Antibodies
• Description: In the absence of viral infection, IRF3 is maintained as a monomer in an autoinhibited state. Phosphorylation by TBK1 and IKBKE disrupts this autoinhibition leading to the liberation of the DNA-binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-IRF3-6C8
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human IRF3 protein was used as the immunogen for the IRF3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This IRF3 antibody is available for research use only.
• UniProt ID: Q14653
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the IRF3 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human MCF-7 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red).

Immunofluorescent staining of human K562 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red).

FACS staining of PFA-fixed human MCF-7 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).

FACS staining of PFA-fixed human K562 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).

FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).

FACS staining of PFA-fixed human U87 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).

FACS staining of PFA-fixed human Raji cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).

Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-6C8). Predicted molecular weight ~47 kDa.

Western blot testing of human MCF7 cell lysate using IRF3 antibody (clone PCRP-IRF3-6C8). Predicted molecular weight ~47 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-6C8). These results demonstrate the foremost specificity of the PCRP-IRF3-6C8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-6C8) as confirmation of integrity and purity.