IDH1 Antibody

• Catalog Number: orb1274348
• Category: Antibodies
• Description: IDH1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45kDa
• Target: IDH1
• UniProt ID: O75874
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IDH1 Antibody: IDH, IDP, IDCD, IDPC, PICD, HEL-216
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts from normal (control) and IDH1 knockout (KO) HeLa cells, using IDH1 antibody (orb1274348) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.