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Catalog Number | orb223933 |
---|---|
Category | Proteins |
Description | Recombinant Human TIMP-2 protein |
Tested applications | SDS-PAGE |
Reactivity | Human |
Purity | > 95% by SDS-PAGE and HPLC analyses. |
Conjugation | Unconjugated |
MW | 21.8 kDa |
Target | Human TIMP-2 |
Source | E. coli |
Biological Activity | Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-ARNH2. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Sterile Filtered White lyophilized (freeze-dried) powder. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
22.6 kDa | |
Human TIMP-2, His Tag (orb257870) is expressed from human 293 cells (HEK293). It contains AA Cys 27 - Pro 220 (Accession # NP_003246.1). |
Greater than 90% as determined by SDS-PAGE. | |
48.5 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2120, Recombinant Human TIMP-2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Cys27-Pro220) of Human TIMP-2 fused with a His Tag at the C-terminal. |
≥90% as determined by SDS-PAGE | |
This protein contains the human TIMP2(Cys27-Pro220) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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