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Catalog Number | orb223841 |
---|---|
Category | Proteins |
Description | Human PDGF B protein |
Tested applications | SDS-PAGE |
Reactivity | Human |
Purity | > 97% by SDS-PAGE and HPLC analyses |
Conjugation | Unconjugated |
MW | 24.8 kDa |
Target | Human PDGF B |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 x 105 IU/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Sterile Filtered White lyophilized (freeze-dried) powder, Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human PDGF B protein
Unconjugated | |
95% | |
15.1 kDa | |
Unconjugated Human PDGF-BB, His, (orb334919) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1). |
Unconjugated | |
90% | |
20.3 kDa | |
Human VEGF-B, His Tag (orb1152444) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Ala 207 (Accession # P49765-1). |
Greater than 85% as determined by SDS-PAGE. | |
28.3 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
14.3 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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