Unconjugated Human PDGF-BB Protein

• Catalog Number: orb334919
• Category: Proteins
• Description: PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
• Reactivity: Human
• Tag: C-6×His & C-Avi
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 15.1 kDa
• Target: PDGF-BB
• Source: Unconjugated Human PDGF-BB, His, (orb334919) is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).
• Expression System: E. coli
• Biological Activity: Immobilized Unconjugated Human PDGF-BB, His, at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.5-16 ng/mL (QC tested).
• Expression Region: Ser 82 - Thr 190
• Endotoxins: 1.0 EU per μg
• NCBI: NP_002599.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 0.085% TFA in 30% ACN
• Alternative names: PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (). The protein has a calculated MW of 15.1 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PDGF-B protein

Line graph illustrates about the sensitivity range of Human PDGF-B protein using different concentrations

Immobilized Unconjugated Human PDGF-BB, His, Avitag (Cat. No. orb334919) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. orb1146967) with a linear range of 0.5-16 ng/mL (QC tested).