Human VEGF-B Protein

• Catalog Number: orb1152444
• Category: Proteins
• Description: Vascular endothelial growth factor B (VEGFB) is also known as VEGF-related factor (VRF), is a secreted O-glycosylated protein, which belongs to the PDGF/VEGF growth factor family. VEGFB is expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. VEGFB is growth factor for endothelial cells. VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. VEGF-B also plays an important role on several types of neurons. It is important for the protection of neurons in the retina and the cerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 20.3 kDa
• Target: VEGF-B
• Source: Human VEGF-B, His Tag (orb1152444) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Ala 207 (Accession # P49765-1).
• Expression System: HEK293
• Biological Activity: Human NRP1, Fc Tag captured on Protein A Chip can bind Human VEGF-B, His Tag with an affinity constant of 23.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Pro 22 - Ala 207
• Endotoxins: 1.0 EU per μg
• NCBI: NP_003368.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: VRF,VEGFL,VEGFB,VEGF-B
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.3 kDa. The protein migrates as 16 kDa and 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human NRP1, Fc Tag (Cat. No. orb257718) captured on Protein A Chip can bind Human VEGF-B, His Tag (Cat. No. orb1152444) with an affinity constant of 23.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).