Human IGF-I Protein

• Catalog Number: orb1146986
• Category: Proteins
• Description: Insulin-like growth factor 1 (IGF-1) is also known as somatomedin C, IGF1A, IGFI, sulfation factor, and is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. IGF-1 binds to at least two cell surface receptors: the Insulin-like growth factor 1 receptor, abbreviated as "IGF1R", and the insulin receptor. The IGF-1 receptor seems to be the "physiologic" receptor - it binds IGF-1 at significantly higher affinity than the IGF-1 that is bound to the insulin receptor. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. Its primary action is mediated by binding to its specific receptor IGF1R, present on many cell types in many tissues. Binding to the IGF1R, a receptor tyrosine kinase, initiates intracellular signaling; IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and proliferation, and a potent inhibitor of programmed cell death. Insulin-like growth factor 1 has been shown to bind and interact with all the IGF-1 Binding Proteins (IGFBPs), of which there are six (IGFBP1-6). Specific references are provided for interactions with IGFBP3, IGFBP4 and IGFBP7.
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 9.5 kDa
• Target: IGF-I
• Source: Human IGF-I, His Tag, premium grade (orb1146986) is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05019-1).
• Expression System: HEK293
• Biological Activity: Biotinylated Human IGF-I R, His, immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).
• Expression Region: Gly 49 - Ala 118
• Endotoxins: 0.1 EU per μg
• NCBI: NP_001104755.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGF-I,IGF1A,somatomedin C,MGF
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 9.5 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Biotinylated Human IGF-I R, His, Avitag immobilized on CM5 Chip can bind Human IGF-I, His Tag, premium grade (Cat. No. orb1146986) with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).