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Catalog Number | orb1806492 |
---|---|
Category | Proteins |
Description | The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation. |
Reactivity | Mouse |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 130.1 kDa |
Target | IGF-I R |
UniProt ID | Q60751 |
Source | Mouse IGF-I R Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # Q60751-1). |
Expression System | HEK293 |
Biological Activity | Immobilized Mouse IGF-I R Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, ,Fc Tag with a linear range of 0.02-0.625 μg/mL (QC tested). |
Expression Region | Glu 31 - His 936 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 130.1 kDa. The protein migrates as 65 kDa,95-120 kDa and 160-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Mouse IGF-I R Protein, Fc Tag (Cat. No. orb1806492) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, Avitag, Fc Tag with a linear range of 0.02-0.625 μg/mL (QC tested).
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