Rat IGF-I R / CD221 Protein

• Catalog Number: orb511247
• Category: Proteins
• Description: The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
• Reactivity: Rat
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 105.5 kDa
• Target: IGF-I R
• Source: Rat IGF-I R, His Tag (orb511247) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # P24062-1).
• Expression System: HEK293
• Biological Activity: Immobilized Rat IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 5-39 ng/mL (QC tested).
• Expression Region: Glu 31 - His 936
• Endotoxins: 1.0 EU per μg
• NCBI: NP_434694.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC1821
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and 24.2 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Rat IGF-I R, His Tag (Cat. No. orb511247) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 5-39 ng/mL (QC tested).