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    Human IGF-I R / CD221 Protein, Fc Tag

    Catalog Number: orb1496214

    DispatchUsually dispatched within 5-10 working days
    $ 3,405.00
    Catalog Numberorb1496214
    CategoryProteins
    DescriptionThe Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mR precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
    ReactivityHuman
    TagC-Fc
    Form/AppearancePowder
    Purity95%
    ConjugationUnconjugated
    MW129.3 kDa
    TargetIGF-I R
    SourceHuman IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).
    Expression SystemHEK293
    Biological ActivityImmobilized Human IGF-I R, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, ,Fc Tag with a linear range of 0.01-0.625 μg/mL (QC tested).
    Expression RegionGlu 31 - Asn 932
    Endotoxins1.0 EU per μg
    Storage-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
    Buffer/PreservativesPBS, pH7.4
    Alternative namesIGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC1821
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    NoteFor research use only
    Application notesThis protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 129.3 kDa. The protein migrates as 70-75 kDa and 105-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Expiration Date6 months from date of receipt.
    Human IGF-I R / CD221 Protein, Fc Tag

    Immobilized Human IGF-I R, Fc Tag (Cat. No. orb1496214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, Avitag, Fc Tag with a linear range of 0.01-0.625 μg/mL (QC tested).

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