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Catalog Number | orb49387 |
---|---|
Category | Proteins |
Description | Recombinant human FGF acidic protein |
Tag | tag free |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 50mM MOPS,100mMNa2SO4,1mM EDTA,pH7.9. |
UniProt ID | P05230 |
Protein Sequence | Phe16-Asp155 |
Source | E.coli |
Expression System | E. coli |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 400mM NaCl, 1mM DTT, pH 8.0, The ED50 for this effect is less than 2 ng/ml, measured in a cell proliferation assay using BALB/c 3T3 cells. |
Alternative names | Fibroblast Growth Factor 1 protein, FGF1 protein, Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
16.5 kDa | |
Human FGF basic, premium grade (orb257225) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4). |
Unconjugated | |
95% | |
16.0 kDa | |
Human FGF acidic, Tag Free (orb257176) is expressed from E. coli cells. It contains AA Phe 16 - Asp 155 (Accession # P05230-1). |
Greater than 85% as determined by SDS-PAGE. | |
17.8 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
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