Human FGF basic Protein

• Catalog Number: orb257225
• Category: Proteins
• Description: FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 16.5 kDa
• Target: FGF basic
• Source: Human FGF basic, premium grade (orb257225) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
• Expression System: E. coli
• Biological Activity: Immobilized Human FGF basic, premium grade at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).
• Expression Region: Pro 143 - Ser 288
• Endotoxins: 0.01 EU per μg
• NCBI: AAI66646.1
• Storage: -20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: FGF2,BFGF,FGFB,FGF basic,HBGF-2
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Wu, Yu-Ting et al. Defining minimum essential factors to derive highly pure human endothelial cells from iPS/ES cells in an animal substance-free system Sci Rep, 5, 9718 (2015)

PubMed: 25864432

SDS-PAGE analysis of Human FGF basic protein

Immobilized Human FGF basic, premium grade (Cat. No. orb257225) at 5 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).