Human Complement C3 Protein

• Catalog Number: orb1087565
• Category: Proteins
• Description: C3 is the major complement component serum. It is mainly synthesized by macrophages and liver. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. It is cleaved into two fragments, C3a and C3b. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 186.8 kDa
• Target: Complement C3
• Source: Human Complement C3, His Tag (orb1087565) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human Complement C3, His Tag at 1 μg/mL (100 μL/well) can bind Human Anti-C3,Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).
• Expression Region: Ser 23 - Asn 1663
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000055.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Complement C3,CPAMD1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 186.8 kDa. The protein migrates as 70 kDa, 100-120 kDa and 160-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human Complement C3, His Tag (Cat. No. orb1087565) at 1 μg/mL (100 μL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).