Biotinylated Human Complement C3 Protein

• Catalog Number: orb1176546
• Category: Proteins
• Description: C3 is the major complement component serum. It is mainly synthesized by macrophages and liver. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. It is cleaved into two fragments, C3a and C3b. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
• Reactivity: Human
• Tag: C-10×His & C-Avi
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Biotin
• MW: 188.5 kDa
• Target: Complement C3
• Source: Biotinylated Human Complement C3, His, (orb1176546) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1).
• Expression System: HEK293
• Biological Activity: Immobilized Biotinylated Human Complement C3, His, at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate can bind Human Anti-C3,Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).
• Expression Region: Ser 23 - Asn 1663
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000055.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Complement C3,CPAMD1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (). The protein has a calculated MW of 188.5 kDa. The protein migrates as 72 kDa,120-125 kDa and 200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human Complement C3, His, Avitag (Cat. No. orb1176546) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).