Human Complement component 3 Protein, Fc Tag (MALS verified)

• Catalog Number: orb1806480
• Category: Proteins
• Description: C3 is the major complement component serum. It is mainly synthesized by macrophages and liver. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. It is cleaved into two fragments, C3a and C3b. C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 211.4 kDa
• Target: Complement component 3
• Source: Human Complement component 3 Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human Complement component 3 Protein, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-C3 antibody with a linear range of 0.1-4 ng/mL (QC tested).
• Expression Region: Ser 23 - Asn 1663
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 211.4 kDa. The protein migrates as 75-80 kDa,160-180 kDa and > 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human Complement component 3 Protein, Fc Tag (Cat. No. orb1806480) at 1 μg/mL (100 μL/well) can bind Anti-C3 antibody with a linear range of 0.1-4 ng/mL (QC tested).