HSP60 Antibody

• Catalog Number: orb2643369
• Category: Antibodies
• Description: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes, and the regulation of protein folding. The mitochondrial and cytosolic localization of HSP60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker for T cell recognition, as well as being involved in a danger signal cascade immune response. HSP60 has been shown to influence apoptosis in tumor cells, and changes in its expression level may serve as a biomarker, as down-regulated HSP60 expression indicates a poor prognosis as well as a risk of tumor infiltration development, especially with regard to urothelial carcinomas. In ovarian cancer, decreased expression of HSP60 correlates with aggressive tumor types, while overexpression is correlated with a better patient prognosis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-HSPD1-1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the HSP60 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HSP60 antibody is available for research use only.
• UniProt ID: P10809
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow testing of PFA-fixed human HeLa cells with HSP60 antibody (clone CPTC-HSPD1-1); Red=isotype control, Blue= HSP60 antibody.

Western blot testing of human HEP-2 and PC3 cell lysate with HSP60 antibody (clone CPTC-HSPD1-1). Predicted molecular weight ~60 kDa.

Immunofluorescent staining of fixed human MCF7 cells with HSP60 antibody (green, clone CPTC-HSPD1-1) and Reddot nuclear stain (red).

IHC staining of FFPE human renal cell carcinoma with HSP60 antibody (clone CPTC-HSPD1-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with HSP60 antibody (clone CPTC-HSPD1-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free HSP60 antibody (clone CPTC-HSPD1-1) as confirmation of integrity and purity.