HSP60 Antibody

• Catalog Number: orb2640578
• Category: Antibodies
• Description: HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Canine, Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein A affinity chromatography
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant HSP60 antibody.
• UniProt ID: P10809
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: HSPD1/2206R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant HSP60 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HeLa lysate with recombinant HSP60 antibody (clone HSPD1/2206R). Predicted molecular weight: ~60 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone HSPD1/2206R) as confirmation of integrity and purity.

IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE dog pancreas tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.