HLA-G Antibody

• Catalog Number: orb1151716
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-G
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HLAG/6686
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Purified His-tagged HLA-G protein was used as the immunogen for the HLA-G antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HLA-G antibody is available for research use only.
• UniProt ID: P17693
• Storage: Aliquot the HLA-G antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HLA-G antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with HLA-G antibody (clone HLAG/6686) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with HLA-G antibody (clone HLAG/6686) at 2 ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Raji cell lysate using HLA-G antibody (clone HLAG/6686).

IHC staining of FFPE human tonsil tissue with HLA-G antibody (clone HLAG/6686) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.