Histone H2A.X (Ab 139) Antibody

• Catalog Number: orb1253553
• Category: Antibodies
• Description: Histone H2A.X (Ab 139) Antibody
• Target: H2AFX
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Immunogen: Histone H2A.X (Ab-139) antibody was raised against a peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
• UniProt ID: P16104
• MW: 15 kDa
• Tested applications: IF, IHC, WB
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H2AX, H2A.X, H2A/X, MCH6, H2AFX, H2a/x, HIST5-2AX
• Note: For research use only
• NCBI: NP_002096.1

Western blot analysis of lysed extracts from HepG2 and 293 cells using Histone H2A.X.

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X. Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X.