His tag antibody

• Catalog Number: orb256380
• Category: Antibodies
• Description: Rat monoclonal antibody to His tag
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D5
• Tested applications: ELISA, FACS, FC, WB
• Isotype: IgG2c
• Immunogen: Purified his tagged Fab fragment.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: His tag (C-term)
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: polyhistidine tag
• Note: For research use only
• Application notes: This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301).
• Expiration Date: 12 months from date of receipt.