6X His Tag antibody

• Catalog Number: orb344412
• Category: Antibodies
• Description: 6X His Tag antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 33D10.D2.G8
• Tested applications: ELISA, IP, WB
• Isotype: IgG1
• Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
• Concentration: 1.09 mg/mL
• Dilution range: ELISA: 1:75,000, IP: 1:200, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: 6X HIS Epitope Tag antibody was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
• Conjugation: Unconjugated
• Storage: Store 6X HIS Tag Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-6X HIS Tag is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-6X His Tag Antibody, anti-HIS, HIS Anti
• Note: For research use only
• Application notes: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, Anti-6X His/6XHIS can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA, SDS-PAGE, and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
• Expiration Date: 12 months from date of receipt.

References

Wang, Pengwei et al. Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery Nat Commun, 10, 5132 (2019)

PubMed: 31723129

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using 6X His Tag antibody

Western blot analysis of 100ng Purified histidine-tagged recombinant protein (Lane1), 200ng E. coli cell lysate containing histidine-tagged expression construct (Lane2), 100ng Purified GST-tagged recombinant protein (Lane3), 100ng Purified FLAG-tagged rec

Western blot analysis of Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged using 6X His Tag antibody