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Catalog Number | orb256373 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to His tag |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3D5 |
Tested applications | ELISA, FACS, FC, WB |
Isotype | Mouse IgG |
Immunogen | Purified his tagged Fab fragment. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | His tag (C-term) |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | polyhistidine tag Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of HEK293 cells with Anti-His tag (C-term) 3D5. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 3D5 (orb256373) (1:200 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
orb256366 (1 µg/ml, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot using Anti-His tag (C-term) antibody 3D5. His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 3D5 (orb256373) at 0.01 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.