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His tag antibody

Catalog Number: orb256373

DispatchUsually dispatched within 5-10 working days
$ 530.00
Catalog Numberorb256373
CategoryAntibodies
DescriptionRabbit monoclonal antibody to His tag
Species/HostMouse
ClonalityMonoclonal
Clone Number3D5
Tested applicationsELISA, FACS, FC, WB
IsotypeMouse IgG
ImmunogenPurified his tagged Fab fragment.
Concentration1 mg/ml
PurityPurified
ConjugationUnconjugated
TargetHis tag (C-term)
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namespolyhistidine tag
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NoteFor research use only
Expiration Date12 months from date of receipt.
His tag antibody

Immunofluorescence staining of HEK293 cells with Anti-His tag (C-term) 3D5. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with His tag expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 3D5 (orb256373) (1:200 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

His tag antibody

orb256366 (1 µg/ml, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Primary incubation was 1 hour. Detected by chemiluminescence.

His tag antibody

Western Blot using Anti-His tag (C-term) antibody 3D5. His tag expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3g protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 3D5 (orb256373) at 0.01 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

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  • His tag antibody [orb256366]

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  • His tag antibody [orb256367]

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  • His tag antibody [orb256368]

    ELISA,  FACS,  FC,  WB

    Mouse

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  • His tag antibody [orb256369]

    ELISA,  FACS,  FC,  WB

    Mouse

    Monoclonal

    Unconjugated

    0.2 mg