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His tag antibody

Catalog Number: orb256366

DispatchUsually dispatched within 5-10 working days
$ 531.00
Catalog Numberorb256366
CategoryAntibodies
DescriptionMouse monoclonal antibody to His tag
Species/HostMouse
ClonalityMonoclonal
Clone Number3D5
Tested applicationsELISA, FACS, FC, WB
IsotypeIgG1
ImmunogenPurified his tagged Fab fragment.
Concentration1 mg/ml
PurityPurified
ConjugationUnconjugated
TargetHis tag (C-term)
StorageStore at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Alternative namespolyhistidine tag
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NoteFor research use only
Application notesThis antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301).
Expiration Date12 months from date of receipt.

WB analysis of of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts using His tag antibody

  • His-Tag antibody [orb763374]

    IF,  IP,  WB

    Mouse

    Monoclonal

    Unconjugated

    100ul, 50ul
  • His-tag antibody [orb323049]

    IF,  IP,  WB

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Cynomolgus IL-10 Protein, His Tag [orb1496244]

    Unconjugated

    90%

    20.6 kDa

    Cynomolgus IL-10 Protein, His Tag (orb1496244) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # P79338).

    20 μg, 50 μg
  • 6X His Tag antibody [orb344412]

    ELISA,  IP,  WB

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • 6X His Tag antibody [orb344413]

    ELISA,  FC,  IHC,  IP,  WB

    Mouse

    Monoclonal

    Unconjugated

    25 μl
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