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Catalog Number | orb1523955 |
---|---|
Category | Antibodies |
Description | HIF1-alpha Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | IP |
Reactivity | Human |
Immunogen | between 776 and 826 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | HIF1-alpha |
UniProt ID | Q16665 |
NCBI | NP_001521.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | HIF1; hypoxia inducible factor 1, alpha subunit (b Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human HIF1-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells treated with 200 µM cobalt chloride (overnight). Antibodies: Affinity purified goat anti-HIF1-alpha antibody orb1523955 used for IP at 6 µg/mg lysate.
FC, IF, IHC-P, WB | |
Bovine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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