HIF1A Antibody

• Catalog Number: orb1262960
• Category: Antibodies
• Description: HIF1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 93 kDa
• Target: HIF1A
• UniProt ID: Q16665
• NCBI: Q16665
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HIF1Alpha Antibody in MDA-MB231 cell line lysates (35 ug/lane)

Western blot analysis of HIF1Alpha Antibody in mouse cerebellum tissue lysates (35 ug/lane)

Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.