HIF1A Antibody

• Catalog Number: orb1262959
• Category: Antibodies
• Description: HIF1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:250For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 93 kDa
• Target: HIF1A
• UniProt ID: Q16665
• NCBI: Q16665
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF
• Note: For research use only
• Application notes: For WB starting dilution is: 1:250For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2 (0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).

Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2 (0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).

HIF1Alpha Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.