H2AX Antibody

• Catalog Number: orb1530714
• Category: Antibodies
• Description: H2AX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ICC-IF - 1:200 - 1:1000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: H2AX
• UniProt ID: P16104
• NCBI: NP_002096.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: H2AX histone; histone H2AX; H2AX; H2A/X; H2A.X; hi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse H2AX by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-H2AX antibody orb1530714 at 0.1 µg/ml.

Detection of mouse H2AX by immunohistochemistry.Sample:FFPE section of mouse renal cell carcinoma.Antibody:Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human H2AX by immunohistochemistry.Sample:FFPE section of human ovarian carcinoma.Antibody:Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human H2AX by IHC.Sample:FFPE section of HCT 116 cell line.Antibody:Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000. Detection:DAB