Histone H2A.X/H2AFX Antibody

• Catalog Number: orb546278
• Category: Antibodies
• Description: Histone H2A.X/H2AFX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Histone H2A.X/H2AFX recombinant protein (Position: S2-T121).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 15 kDa
• UniProt ID: P16104
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-H2AFX antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of H2AFX using anti-H2AFX antibody.Lane 1:human HepG2 cell;2:human Caco-2 cell.

WB analysis of H2AFX using anti-H2AFX antibody.Lane 1:human Caco-2 cell; 2:human HepG2 cell.

IHC analysis of H2AFX using anti-H2AFX antibody.H2AFX was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of H2AFX using anti-H2AFX antibody.H2AFX was detected in paraffin-embedded section of rat small intestine tissues.

IHC analysis of H2AFX using anti-H2AFX antibody.H2AFX was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of H2AFX using anti-H2AFX antibody.H2AFX was detected in paraffin-embedded section of human rectal cancer tissues.

IHC analysis of H2AFX using anti-H2AFX antibody.H2AFX was detected in paraffin-embedded section of mouse brain tissues.

IHC analysis of H2AFX using anti-H2AFX antibody.H2AFX was detected in paraffin-embedded section of mouse small intestine tissues.