H2AX Antibody

• Catalog Number: orb1530712
• Category: Antibodies
• Description: H2AX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IHC - 1:40 to 1:200. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: H2AX
• UniProt ID: P16104
• NCBI: NP_002096.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: H2AX histone; histone H2AX; H2AX; H2A/X; H2A.X; hi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse H2AX by western blot.Samples: Whole cell lysate from F9, RenCa, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-H2AX antibody at 0.04 µg/ml.

Detection of human H2AX by western blot.Samples: Whole cell lysate from A-549, LNCaP, MCF-7, and U2OS cells. Antibody: Affinity purified rabbit anti-H2AX antibody at 0.04 µg/ml.

Detection of human H2AX by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:5, 000 (0.2µg/ml).