H2AX Antibody

• Catalog Number: orb1524090
• Category: Antibodies
• Description: H2AX Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:1,0000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: H2AX
• UniProt ID: P16104
• NCBI: NP_002096.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: H2AX histone; histone H2AX; H2AX; H2A/X; H2A.X; hi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human H2AX by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and K-562cells prepared using RIPA lysis buffer. Antibody: Affinity purified goat anti-H2AX antibody orb1524090 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse H2AX by immunohistochemistry.Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified goat anti-H2AX (Cat. No. orb1524090) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human H2AX by immunohistochemistry.Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified goat anti-H2AX (Cat. No. orb1524090) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB