H2AFX Antibody

• Catalog Number: orb1253452
• Category: Antibodies
• Description: H2AFX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Immunogen: Histone H2A.X (Phospho-Ser139) antibody was raised against a peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 15 kDa
• Target: H2AFX
• UniProt ID: P16104
• NCBI: NP_002096.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: H2AX, H2A.X, H2A/X, HNF3B, TCF3B, H2AFX, H2a/x, HI
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from HT29 cells untreated (Lane 1) or treated with UV (lane 2) using Histone H2A.X (Phospho-Ser139).

Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X (Phospho-Ser139).