GPSM2 antibody

• Catalog Number: orb19420
• Category: Antibodies
• Description: Goat polyclonal antibody to GPSM2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 75.8
• Target: GPSM2
• Entrez: 29899
• Protein Sequence: NRLKGKKYKTNSSTK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GPSM2 antibody, anti G-protein signalling mod
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in Human Brain (Cerebellum) and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Recommended concentration: 0.01-0.03 μg/ml. An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19420 (0.01µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19420 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.