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Catalog Number | orb1274232 |
---|---|
Category | Antibodies |
Description | GPC3 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | GPC3/1534R |
Tested applications | FC, IF, IHC-P |
Reactivity | Human, Rat |
Isotype | IgG, kappa |
Immunogen | A partial human protein was used as the immunogen for the recombinant Glypican-3 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | GPC3 |
UniProt ID | P51654 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7 Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human fetal liver tissue with recombinant Glypican-3 antibody (clone GPC3/1534R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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