GLS2 Mouse Monoclonal Antibody

• Catalog Number: orb783358
• Category: Antibodies
• Description: GLS2 Mouse Monoclonal Antibody
• Target: GLS2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human GLS2.
• UniProt ID: Q9UI32
• MW: 65 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: Y09A1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: breast cell glutaminase; GLSL_HUMAN; glutaminase 2
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue sections), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLS2) Monoclonal Antibody, Unconjugated (orb783358) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human brain tissue lysates, Lane 2: Mouse liver tissue lysates, Primary: Anti-GLS2 (orb783358) at 1/1000~2000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.