GAPDH antibody

• Catalog Number: orb705357
• Category: Antibodies
• Description: Mouse monoclonal antibody to GAPDH
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein corresponding to GAPDH.
• Dilution range: WB: 1:3000-1:10000, IHC-P: 1:50-1:100, IF: 1:50-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% rAlbumin, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GAPDH
• Entrez: 100042025, 2597, 24383
• UniProt ID: P16858, P04406, P04797
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% rAlbumin, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-GAPD antibody, anti-Glyceraldehyde-3-phosphat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GAPDH expression in Hela (A), mouse brain (B), mouse spleen (C), rat liver (D), rat lung (E) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)

Immunohistochemical analysis of GAPDH staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GAPDH staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.