GAPDH-Loading Control antibody

• Catalog Number: orb500708
• Category: Tools
• Description: GAPDH-Loading Control antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ICC, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Monkey, Rabbit, Sheep
• Reactivity: Hamster, Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: Recombinded Human GAPDH
• Concentration: 1mg/ml
• Dilution range: WB=1:10000-1:100000, IHC-P=1:100-500, ICC=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 38 kDa
• Target: GAPDH-Loading Control
• UniProt ID: P04406
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: 38 kDa BFA-dependent ADP-ribosylation substrate; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Althenayyan, Saleh et al. Alternatively Spliced Isoforms of MUC4 and ADAM12 as Biomarkers for Colorectal Cancer Metastasis J Pers Med, 13, 135 (2023)

PubMed: 36675796

Western blot:Lane 1:Human HEK293 cell;Lane 2:Human Hela cell;Lane 3:Human SH-SY5Y cell;Lane 4:Mouse NIH/3T3 cell;Lane 5:Hamster CHO cell;Lane 6:Rat Brain;Primary:Anti-GAPDH at 1:200000 dilution.

Western blot analysis using Anti-GAPDH antibody at 1:1000 dilution.

ICC analysis of Hela cell using GAPDH-Loading Control monoclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of mouse brain using GAPDH-Loading Control Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of rat brain using GAPDH-Loading Control Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of Human kidney using GAPDH-Loading Control Monoclonal Antibody at 1:200 dilution.

Western blot:Cerebrum (Rat);Primary:Anti-GAPDH at 1:2000-1:20000 dilution.