GAPDH antibody

• Catalog Number: orb500826
• Category: Tools
• Description: GAPDH antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human GAPDH full length protein
• Concentration: 1mg/ml
• Dilution range: WB=1:10000-200000, IHC-P=1:100-500, ICC=1:100, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 38 kDa
• Target: GAPDH (Loading Control)
• UniProt ID: P04406
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: 38 kDa BFA-dependent ADP-ribosylation substrate; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Duan, Yang-Yang et al. Microtubule Stabilization Promotes Microcirculation Reconstruction After Spinal Cord Injury J Mol Neurosci, 71, 583-595 (2021)

PubMed: 32901373

ICC analysis of A549 cell using GAPDH (Loading Control) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of rat colon using GAPDH Loading Control Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of Human kidney using GAPDH Loading Control Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse brain using GAPDH Loading Control Polyclonal Antibody at 1:200 dilution.

Western blot:H9C2(Rat) Cell;Primary:Anti-GAPDH at 1:2000 dilution.

Western blot:293T(Human) Cell;Primary:Anti-GAPDH at 1:1000 dilution.

Western blot analysis using Anti-GAPDH antibody at 1:1000 dilution.