GAPDH antibody

• Catalog Number: orb18774
• Category: Antibodies
• Description: Goat polyclonal antibody to GAPDH
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine
• Dilution range: ELISA: 1:128000, WB: 0.001-0.003 μg/ml, IHC-P: 2.5 μg/ml, IF/ICC: 10μg/ml
• Conjugation: Unconjugated
• MW: 36.1
• Target: GAPDH (C Terminus) Loading Control
• Entrez: 2597
• Protein Sequence: HQVVSSDFNSDT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GAPDH antibody, anti glyceraldehyde-3-phospha
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

References

Hain, Anika et al. IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells Sci Rep, 8, 3217 (2018)

PubMed: 29453458

Immunohistochemical staining of Human Pancreas using GAPDH antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using GAPDH antibody.

Immunofluorescence analysis of paraformaldehyde fixed A549 cells using GAPDH antibody.

Western blot analysis of Human Liver (A), Tonsil (B) and (0.3ug/ml) Rat Brain (C) lysate using GAPDH antibody

Western blot analysis of HEK293 (A) and HeLa (B) cell lysate using GAPDH antibody