GAPDH Antibody

• Catalog Number: orb389477
• Category: Antibodies
• Description: GAPDH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Gallus, Human, Monkey, Mouse, Rat, Zebrafish
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36053 MW
• UniProt ID: P04406
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;1.2
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of GAPDH using anti-GAPDH antibody.Lane 1:human HeLa cell; 2:human CACO-2 cell;3:human CCRF-CEM cell;4:rat brain tissue;5:rat liver tissue;6:mouse brain tissue;7:mouse liver tissue.

IHC analysis of GAPDH using anti-GAPDH antibody. GAPDH was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.

IHC analysis of GAPDH using anti-GAPDH antibody. GAPDH was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue.

IHC analysis of GAPDH using anti-GAPDH antibody. GAPDH was detected in a paraffin-embedded section of human ovarian serous cancer tissue.