GAPDH Antibody

• Catalog Number: orb1524011
• Category: Antibodies
• Description: GAPDH Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 175 and 225
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:500 - 1:2,500; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GAPDH
• UniProt ID: P04406
• NCBI: NP_002037.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: GAPD; peptidyl-cysteine S-nitrosylase GAPDH; Oct1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse GAPDH by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , Jurkat (50µg) , and mouse NIH 3T3 (50µg) cells. Antibodies: Affinity purified goat anti-GAPDH antibody orb1524011 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GAPDH by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (Cat. No. orb1524011) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB