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Catalog Number | orb1238400 |
---|---|
Category | Antibodies |
Description | GAPDH Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 12D3D5 |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH. |
Concentration | 1 mg/mL |
Dilution range | GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 37 kDa Observed: 36 kDa |
Target | GAPDH |
UniProt ID | P04406 |
NCBI | NP_002037 |
Storage | GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. |
Buffer/Preservatives | GAPDH antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, G Read more... |
Note | For research use only |
Application notes | GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of GAPDH in multiple cell and tissue lysates with GAPDH antibody at 1 μg/ml. Lanes 1-23, 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, human heart, rat heart, human liver, mouse liver, rat liver, rabbit liver, respectively.
ICC, IHC-P, IP, WB | |
Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-P, WB | |
Canine, Gallus, Monkey, Rabbit, Sheep | |
Hamster, Human, Mouse, Porcine, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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