Gamma-enolase antibody

• Catalog Number: orb1474030
• Category: Antibodies
• Description: Mouse monoclonal antibody to Gamma-enolase
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human Gamma-enolase. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/100 - 1/500)
• Form/Appearance: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ENO2
• Entrez: 2026
• UniProt ID: P09104
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Gamma-enolase; 2-phospho-D-glycerate hydro-lyase;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Gamma-enolase expression in Jurkat (A), CEM (B), U251 MG (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunohistochemical analysis of Gamma-enolase staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.